락탐 합성 신규 효소 최초 발굴

Soo-Jin Yeom, Moonjeong Kim, Kil koang Kim, Yaoyao Fu, Eugene Rha, Sung-Hyun Park, Hyewon Lee, Haseong Kim, Dae-Hee Lee, DongMyung kim and Seung-Goo Lee. A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts. Nature Communications. 5053 (2018)

MVA 경로의 이소프렌 감지 바이오센서 개발

Kim, S. K., Kim, S., Subhadra, B., Woo, S. G., Rha, E., Kim, S. W., ... & Lee, S. G. (2018). A genetically encoded biosensor for monitoring isoprene production in engineered Escherichia coli. ACS synthetic biology.

Cellobiose 감지 전사인자 CelR 구조 규명

Yaoyao Fu, Soo-Jin Yeom, Kil Koang Kwon, Jungwon Hwang, Haseong Kim, Eui-Jeon Woo, Dae-Hee Lee, Seung-Goo Lee. (2018-07-31) Structural and functional analyses of the cellulase transcription regulator CelR. FEBS Letters. doi: 10.1002/1873-3468.13206. [Epub ahead of print] IF2.999 ISSN: 0014-5793(print), 1873-3468

고속 탐색용 유전자회로 기반 단백질 신규 활성 도입

Evolution of enzymes with new specificity by high-throuput screening using DmpR-based genetic circuiuts and multiple flow cytometry rounds Kil Koang Kwon, Dae-Hee Lee, Su Jin Kim, Su-Lim Choi, Eugene Rha, Soo-Jin Yeom, Bindu Subhadra, Jinhyuk Lee, Ki Jun Jeong, Seung-Goo Lee Scientific Reports (2018) 8(1), 2659 DOI: 10.1038/s41598-018-20943-8

Cellulase 고속탐색용 유전자회로 개발 및 신규 효소 발굴

Development of a novel cellulase biosensor that detects crystalline cellulose hydrolysis using a transcriptional regulator Kil Koang Kwon, Soo-Jin Yeom, Dae-Hee Lee, Ki Jun Jeong, Seung-Goo Lee Biochemical and Biophysical Research Communications (2018) 495(1), 1328-1334 DOI: 10.1016/j.bbrc.2017.11.157

신규 CRISPR-Cpf1 (Type V-A) 발굴 및 구동

Efficient Transcriptional Gene Repression by Type V-A CRISPR-Cpf1 from Eubacterium eligens Seong Keun Kim, Haseong Kim, Woo-Chan Ahn, Kwang-Hyun Park, Euijeon Woo, Dae-Hee Lee, and Seung-Goo Lee ACS synthetic Biology (2017) 6(7), 1273-1282 DOI:10.1021/acssynbio.6b00368

CRISPRi 기반 대사흐름 제어 및 유용 대사물질 생산

CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli Seong Keun Kim, Wonjae Seong, Gui Hwan Han, Dae-Hee Lee, and Seung-Goo Lee Microbial Cell Factories (2017) 16, 188 DOI:10.1186/s12934-017-0802-x.

메타볼론 기반 효소 활성 개선 기술

Controlled Aggregation and Increased Stability of β-Glucuronidase by Cellulose Binding Domain Fusion Soo-Jin Yeom, Gui Hwan Han, Moonjung Kim , Kil Koang Kwon, Yaoyao Fu, Haseong Kim, Hye-Won Lee, Dae-Hee Lee, Heungchae Jung, and Seung-Goo Lee PLoS One (2017) 12(1):e0170398 DOI: 10.1371/journal.pone.0170398.eCollection

메타볼론 기반 고효율 효소 연속반응 구현

Leucine zipper-mediated targeting of multi-enzyme cascade reactions to inclusion bodies in Escherichia coli for enhanced production of 1-butanol. Gui Hwan Han, Wonjae Seong, Yaoyao Fu, Paul K. Yoon, Seong Keun Kim, Soo-Jin Yeom, Dae-Hee Lee, Seung-Goo Lee Metabolic Engineering (2017) 40, 41-49 DOI: 10.1016/j.ymben.2016.12.012

센서 유전자회로 기반 고효율 맞춤형 미생물 검색 플랫폼

A cell-cell communication-based screening system for novel microbes with target enzyme activities Haseong Kim, Eugene Rha, Wonjae Seong, Soo-Jin Yeom, Dae-Hee Lee, Seung-goo Lee ACS Synthetic Biology (2016) 18;5(11):1231-1238

한국생명공학연구원에 위치한 본 연구실에서는 효소공학, 단백질공학, 대사공학, 시스템생물학 분야의 전문가들과 함께 합성생물학 기반의 원천 기술을 개발하고 관련된 실용화 연구를 수행하고 있습니다.